CELL ADHESION

Computer Engineering electronics Engineering Civil Engineering

The study of cell-surface interactions is important for several different applications. While the effects of surfaces on cell proliferation, migration, growth and genes expression are accessible via traditional microscopy and labeling techniques, the determination of cell adhesion remains a major challenge. In the context of the European project Newbone, working on development of a new generation of non-metallic biomimetic bone implants, cell adhesion is also at the center of the preoccupations. The objective is not only to observe modifications of adhesion properties on different surfaces but mainly to develop some strategies of cell adhesion measurements in order to understand the mechanisms responsible of cell adhesion modulations. Atomic force microscopy, and particularly force spectroscopy are used to directly measure the cell adhesion. The JPK Nanowizard II is a bioAFM allowing to manipulate, to stretch and to detach cells with a z-range of 100 ?m. loading..